Dr. Stefan Huber
Floor system, not bench system. Minimal floor space required: 90 cm x 70 cm (W/D).
Laboratory conditions preferred, non-laboratory conditions possible. Room should be climatized to any temperature between 18 - 27 °C ± 2°C. Short temperature changes in excess of ± 2°C may induce slight baseline drifts. Do not expose LC-OCD to direct sunlight.
For operation nitrogen 4.0 or better is required. Supply either on-site (pressure bottle) or central.
Nitrogen consumption only 200 sccm/min. (50L-bottle lasts for about 4 weeks continuous operation). Nitrogen must be reduced to 0.5 bar approx. Pressure reducer must have a Swagelok 1/8 inch outlet. Teflon or SS tubing to LC-OCD can be pre-mounted or will be carried out by us.
Note: Conventional Nitrogen Generators with PSA often do not supply the quality required, due to fluctuations in CO2 content! For such systems additional purification steps may be necessary. In case of uncertainty we recommend to provide a 10L-bottle for testing.
220 - 240 V, 50 Hz are required. Consumption is only 350 W/hr. At least 3 sockets are required, one for LC-OCD, one for Laptop and one for waste water pump. Maximal distance of sockets to LC-OCD is 5 m. Customer is kindly asked to provide at least 2 plugs in case plugs have to be changed.
Waste produced is mild phosphate buffer pH 5, about 5 g/L. Waste is exported with external pump either to a nearby container, or better with a tubing to a sink. We kindly ask customer to provide silicon or PP tubing for connection of waste pump to sink.
Please try to make the lab where LC-OCD is positioned to a TOC-sensitive area. This includes the following:
a) No organic solvents must be used in the lab. If HPLC-systems with organic solvents are present then special precautions (e.g.
hoods or carbon filters for HPLC mobile phase flasks) should be taken.
b) No felt pens are allowed in the lab.
c) No perfumes, no creams, no hair conditioner for personal working in the lab
Please note: Volatile organics do not impair the performance of the LC-OCD system, but organics may contaminate samples.
7) List of chemicals needed for Operation of LC-OCD
We kindly ask Customers to have the following chemicals on stock prior to installation of system. Systems will be shipped with smaller amounts of chemicals for 1-month operation
a) Mobile Phase:
1) Potassium-dihydrogenphosphate (e.g. Fluka / Riedel de Haen 30407 (1 kg)
2) Sodium-hydrogenphosphate-dihydrate (e.g. Fluka / Riedel de Haen: 30412 (500g)
b) Acidification solution:
3) Orthophoshoric Acid 85 % (e.g. Fluka / Riedel de Haen 79620-1L (1 Liter)
4) Potassium Peroxodisulfate (e.g. Fluka 60489).
c) Calibration of Detectors
5) Potassium Hydrogen Phthalate (e.g. Fluka 60359).
6) Potassium Nitrate (e.g. Fluka / Riedel de Haen 31263-100g (100 g)
d) Calibration of Molecular masses:
7) Suwannee River Humic Acids Standard II, Catalog # 25101 H
8) Suwannee River Fulvic Acids Standard II, Catalog # 25101 F
Order d) from International Humic Substances Society (order samples online). (Small amounts of Humic Substances will be shipped
with the LC-OCD system.)
Please have the following materials on stock:
1 x 5L volumetric flask
1 x 5L Schott-flask amber (for mobile phase)
1 x 5L Schott flask transparent (for acidification solution)
For cleaning of vials:
We recommend 3 basins about 10 L each for chemical cleaning.
Alternative: A kiln or oven for heating up vials to 550 °C.
Also standard laboratory equipment for dilution and weighting-in is required.
We recommend to have at least 2 persons being instructed.
We suggest to have one person identified as main responsible person or principal operator. This person has to make sure that system is running well and also has to organise and train supporting personal in case of absence.